Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
40, Number 2, February 2002, pp. 113-118
A High-Pressure Liquid Chromatographic–Tandem Mass Spectrometric
Method for the Determination of Ethambutol in Human Plasma, Bronchoalveolar
Lavage Fluid, and Alveolar Cells
John E. Conte, Jr.[1],[2],[3],
Emil Lin[4], Yeping Zhao[4], and Elisabeth Zurlinden[1]
[1]Department of Epidemiology and Biostatistics, Infectious Diseases Research
Laboratory,
[2]Department of Medicine,
[3]Department of Microbiology and Immunology, and
[4]Department of Biopharmaceutical Sciences, University of California, San Francisco,
350 Parnassus Avenue, Suite 507, San Francisco, CA 94117
A technique is presented for the specific and sensitive determination
of ethambutol concentrations in plasma, bronchoalveolar lavage (BAL), and alveolar
cells (AC) using a high-pressure liquid chromatographic (HPLC)–tandem mass
spectrometric (MS–MS) method. The preparation of samples requires a deproteinization
step with acetonitrile. The retention times for ethambutol, neostigmine bromide,
and propranolol are 2.0, 1.4, and 1.1 min, respectively, with a total run time
of 2.8 min. The detection limits for ethambutol are 0.05 µg/mL for plasma
and 0.005 µg/mL for the BAL supernatants and AC suspensions. The assay
has excellent performance characteristics and has been used to support a study
of the intrapulmonary pharmacokinetics of ethambutol in human subjects.
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