Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
40, Number 2, February 2002, pp. 107-112
Temperature Effect on Peak Width and Column Efficiency in Subcritical
Water Chromatography
Yu Yang[1], Lori J. Lamm[1],
Ping He[1], and Toru Kondo[2]
[1]Department of Chemistry, East Carolina University, Greenville, NC 27858 and
[2]Fuji Silysia Chemical Ltd., Kasugai-Shi, Aichi-Ken, 487-0013, Japan
Subcritical water has been recently employed as the mobile phase
to eliminate the use of organic solvents in reversed-phase liquid chromatography.
Although the influence of temperature on retention in subcritical water chromatography
has been reported, the temperature effect on peak width and column efficiency
has not yet been quantitatively studied. In this work, several polar and chlorinated
compounds are separated using pure subcritical water on Zorbax RX-C8, PRP-1
(polystyrene–divinylbenzene), Hypersil ODS, and ZirChrom-polybutadiene
columns. Isothermal separations are performed at temperatures ranging from 60°C
to 160°C. The retention time and peak width of analytes are reduced with
increasing temperature. However, the column efficiency is either improved or
almost unchanged with the increasing temperature in the low-temperature range
(lower than the 100°C to 120°C range), but it is decreased when temperature
is further raised in the high-temperature range (higher than the 100°C to
120°C range). Therefore, a maximum in column efficiency is obtained at temperatures
within the 100°C to 120°C range in most cases.
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