Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
40, Number 2, February 2002, pp. 101-106
Evaluation of Select Variables in the Ion Chromatographic Determination
of F–, Cl–, Br–, NO3, SO4-2, and PO4-3 in Serum
Samples
Z. Benzo[1], A. Escalona[3],
J. Salas[1], C. Gómez[1], M. Quintal[1], E. Marcano[1], F. Ruiz[1], A.
Garaboto[1], and F. Bartoli[2]
[1]Centro de Química and
[2]Dpto. de Biologia estructural, Instituto Venezolano de Investigaciones Científicas,
IVIC, Apdo. Postal 21827, Caracas 1020-A, Venezuela and
[3]Centro de Química Analítica, Facultad de Ciencias, Universidad
Central de Venezuela, Apdo. Postal 47102, Caracas 1041-A, Venezuela
A full experimental design at two levels is applied for the estimation of the significance of select factors that may influence the ion chromatography (IC) determination of F, Cl, Br, NO3, SO4-2, and PO4-3 in serum samples. The factors studied are various sample deproteinization procedures, eluent composition, and flow rates. Deproteinization using either acetonitrileNaOH or ultrafiltration can be used in order to obtain a significant protein removal before IC analysis; however, the former is recommended because it is less time-consuming and cheaper. Better resolution is obtained when a sodium hydroxide solution is used as the eluent. There is no influence of the sample’s deproteinization procedures on the chromatographic resolution.
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