Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
40, Number 7, August 2002, pp. 409-416
Isolation of a New Member of the Ecdysteroid Glycoside Family:
2-Deoxy-20-hydroxyecdysone 22-O-b-D-Glucopyranoside
Mária Báthori[1], Zita Pongrácz[1], Gábor
Tóth[2], András Simon[2], Lili Kandra[3], Zoltán Kele[4],
and Róbert Ohmacht[5]
[1]Department of Pharmacognosy, University of Szeged, H-6720 Szeged, Eötvös
utca 6, Hungary;
[2]Institute for General and Analytical Chemistry, Technical University of Budapest,
Budapest, Hungary;
[3]Institute of Biochemistry, University of Debrecen, Debrecen, Hungary;
[4]Department of Medical Chemistry, University of Szeged, Szeged, Hungary; and
[5]Institute of Medicinal Chemistry and Biochemistry, University of Pécs,
Pécs, Hungary
A new ecdysteroid glycoside, 2-deoxy-20-hydroxyecdysone 22-O-b-D-glucopyranoside,
is isolated from the herb Silene italica ssp. nemoralis (Waldst. and Kit.) Nyman.
The compound is purified with multistep chromatography, such as classical column
chromatography on alumina and droplet countercurrent distribution. Also, it
is expanded using twice low-pressure reversed-phase liquid column chromatography.
Chromatography in four steps results in the purified 2-deoxy-20-hydroxyecdysone
22-O-b-D-glucopyranoside.
Two other ecdysteroids have also been separated, including the formerly identified
integristerone A and 24(28)-dehydromakisterone A.
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