Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
40, Number 7, August 2002, pp. 369-376
Comparison of Reversed-Phase Liquid Chromatographic Methods for
the Separation of New Quinolones
Byoung-Hyoun Kim, Nak Hee Choi, and Jong Hoa Ok
Analytical R&D Center, LG Chemical Research Park, LG Chemical Ltd., 104-1,
Moonji-dong, Yusong-gu, Daejon, 305-380, South Korea
Reversed-phase high-performance liquid chromatography is widely
used in the analysis of drug substances and their metabolites. The interaction
of quinolones with residual silanol in a silica-based C18 stationary
phase causes peak broadening and bad peak shapes and makes it hard to resolve
the peak separations. This unusual interaction is studied and finally can be
removed by masking the residual silanol of a silica-based C18 stationary
phase, then good peak separation is achieved. We have chosen four new quinolones
and ciprofloxacin and improved the peak shapes by optimizing the pH of the eluent
and the quantity of the additive (N,N-dimethyloctylamine, approximately 0–40mM)
in the monomeric C18 stationary phase. The elution behavior of quinolones
in the polymeric C18 stationary phase is compared with that in the
monomeric C18 stationary phase under the same eluent condition. Good
peak symmetry and a high plate number are achieved by this technique, which
are hardly obtained with the conventional silica-based C18 stationary
phase. Based on these results, we present data of the influence of the eluent
composition such as pH, buffer, and additive concentration on the peak shape.
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