Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
40, Number 4, April, pp. 219-223
Determination of Lamotrigine Simultaneously with Carbamazepine,
Carbamazepine Epoxide, Phenytoin, Phenobarbital, and Primidone in Human Plasma
by SPME–GC–TSD
M.E.C. Queiroz, S.M. Silva,
and D. Carvalho
University of Ribeirão Preto, Department of Pharmaceutical Science, Ribeirão
Preto, SP, Brazil
F.M. Lanças*
University of São Paulo, Institute of Chemistry of São Carlos, São
Carlos, SP, Brazil
A simple and rapid analytical method is presented for the determination
of lamotrigine simultaneously with primidone, carbamazepine, carbamazepine epoxide,
phenobarbital, and phenytoin in human plasma using solid-phase microextraction
(SPME) and gas chromatography with thermionic specific detection. The best conditions
for the SPME procedure is established as following: direct extraction on a 65-µm
Carbowax–divinylbenzene fiber; 1.0 mL of a sample plasma matrix modified
with 15% NaCl and 3 mL of a potassium phosphate buffer (pH 7.0); extraction
temperature at 30°C; and stirring at a rate of 2500 rpm for 15 min. The
method shows good linearity between 0.05 and 40.0 µg/mL with regression
coefficients ranging between 0.9965 and 0.9995 and a coefficient of variation
of the points of the calibration curve lower than 10%. The lowest limit of quantitation
for the plasma-investigated drugs varies from 0.05 to 0.20 µg/mL, according
to the drug. The proposed method is sensitive enough to work into subtherapeutic
and therapeutic concentrations, being that it is applied in pharmacokinetic
studies and patient routine therapeutic drug monitoring.
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