Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
40, Number 4, April, pp. 214-218
Assay of TNP-470 and Its Two Major Metabolites in Human Plasma
by High-Performance Liquid Chromatography–Mass Spectrometry
Carl T. Whalen[1], Glenn
D. Hanson[1], Kathy J. Putzer[1], Michael D. Mayer[2], and Darcy J. Mulford[2]
[1]Covance Laboratories Inc., 3301 Kinsman Blvd., Madison, WI 53704 and [2]TAP
Pharmaceutical Products, Inc., 675 N. Field Drive, Lake Forest, IL 60045
In this study, a sensitive, specific assay for the determination
of TNP-470 and its two major metabolites M-IV (also know as AGM-1883) and M-II
in human plasma is reported. The assay involves liquid–liquid extraction
of acidified plasma followed by reversed-phase high-performance liquid chromatography–atmospheric
pressure chemical ionization–tandem mass spectrometry. A liquid–liquid
extraction using an organic solvent mixture (methyl-tert-butyl-ether–hexane,
1:1, v/v) is used in place of solid-phase extraction because it provides consistent
recoveries for all analytes, including the internal standard. Retention times
for the analytes and internal standard are less than 7 min. Within- and between-day
precision is £ 6.5% and £ 13.3% relative standard deviation, respectively,
for the three analytes. The lower limits of quantitation are 0.25, 0.5, and
1.0 ng/mL for TNP-470, M-IV, and M-II, respectively.
Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
This
article is available in its entirety by fax for $40.00.
Visa, MasterCard & Amex accepted.
To
order electronicallyclick here or call: 847-647-2900 ext. 1323 or fax request to: 847-647-1155.
Please
indicate JCS volume and issue along with
page numbers.
For permission to reuse material from Journal of Chromatographic Science, please access www.copyright.com or contact the Copyright Clearance Center, Inc. (CCC), 222 Rosewood Drive, Danvers, MA 01923, 978-750-8400. CCC is a not-for-profit organization that provides licenses and registration for a variety of uses.
