Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
40, Number 4, April, pp. 198-200
An HPLC Method for the Determination and Pharmacokinetic Study
of Cinnamic Acid in the Plasma of Rats Having Taken the Traditional Chinese
Medicinal Preparation Ling-Gui-Zhu-Gan Decoction
Zonghua Song, Kaishun Bi*,
and Xu Luo
Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, Liaoning
Province, P.R. China
A high-performance liquid chromatographic method for the determination
and pharmacokinetic study of cinnamic acid in the plasma of rats after having
been administrated orally with the traditional Chinese medicinal (TCM) preparation
Ling-Gui-Zhu-Gan decoction is established. Plasma samples taken from rats are
acidified with hydrochloric acid and extracted with ethyl acetate. Separation
of the main effective constituent cinnamic acid is accomplished on a C18 stationary
phase and a mobile phase of methanol–acetonitrile–2% glacial acetic
acid (10:22:70, v/v) with a UV detector setting at 254 nm. After validation,
the method is used to take a limited view of pharmacokinetic profiles of the
TCM preparation of the Ling-Gui-Zhu-Gan decoction.
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