Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
39, Number 9, September 2001, pp. 370-374
Separation
and Determination of Ephedrine Alkaloids and Tetramethylpyrazine in Ephedra
sinica Stapf by Gas ChromatographyMass SpectrometryHong-Xia
Li, Ming-Yu Ding, Kun Lv, and Jian-Yuan Yu
Tsinghua University, Beijing, 100084, China
A simple, sensitive, and reliable method using gas chromatography
(GC)–mass spectrometry (MS) is developed for the simultaneous determination
of ephedrine alkaloids and 2,3,5,6-tetramethylpyrazine (TMP) in Ephedra sinica
Stapf. The sample is extracted with ethyl ether and submitted to GC–MS
for identification and quantitation without derivatization. The column used
for GC is an HP-5 (30.0 m x 250 µm x 0.25 µm, 5% phenyl methyl siloxane),
and the carrier gas is helium. The detection limits for ephedrine, pseudoephedrine,
and TMP are 0.4 ng, 0.7 ng, and 0.02 ng (signal-to-noise ratio of 3), respectively.
The reproducibility of the total procedure is proved to be acceptable (RSD <
2%), and the recoveries are above 93%.
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