Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
39, Number 10, October 2001, pp. 441-444
The Presence of Three Isoflavonoid Compounds in Psoralea corylifolia
Yun-Ting Hsu[1], Chih-Jen Wu[2], Jing-Ming Chen[3], Yuh-Cheng
Yang[1],[4], and Sung-Yuan Wang[1]
[1]Department of Medical Research and [2]Department of Internal Medicine, Mackay
Memorial Hospital, Taipei, Taiwan; [3]Taipei Chinese Medicine Doctor Association,
Taiwan; and [4]Department of Obstetrics and Gynecology, Mackay Memorial Hospital
and Taipei Medical College, Taipei, Taiwan
The optimization of a high-performance liquid chromatographic
method to determine three isoflavonoids (daidzein, genistein, and biochanin
A) in the fruit of Psoralea corylifolia is developed and validated. Dried psoralea
fruit powder is extracted with aqueous methanol followed by the hydrolysis of
the analytes’ conjugated glycosides with hydrochloric acid. The HPLC assay
is performed on a reverse-phase C18 column with gradient elution using acetonitrile
and 10% acetic acid as the mobile phase at a flow rate of 0.8 mL/min. Flavone
is used as the internal standard and the substances are detected at 260 nm.
Calibrations are linear (correlation coefficient Ž 0.995) for all three analytes.
The limits of detection are 0.01 µg/mL for daidzein and genistein and
0.1 µg/mL for biochanin A. The overall intra- and interassay precision
range from 2.5% to 4.9% and from 0.5% to 4.7%, respectively. The method proved
to be sensitive, specific, accurate, and precise for the determination of daidzein,
genistein, and biochanin A in Psoralea corylifolia.
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