|
Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
39, Number 10, October 2001, pp. 425-430
Quantitative Determination of the Loop Diuretic Bumetanide in
Urine and Pharmaceuticals by High-Performance Liquid Chromatography with Amperometric
Detection
M.J. Legorburu[1], R.M. Alonso[1], R.M. Jiménez[1], and
E. Ortiz[2]
[1]Departamento de Química Analítica, Facultad de Ciencias, Universidad
del País Vasco/EHU., Apdo. 644, 48080 Bilbao, Spain and [2]Departamento
de Especialidades Médico-Quirúrgicas, Facultad de Medicina, Universidad
del País Vasco/EHU., Apdo. 644, 48080 Bilbao, Spain
A high-performance liquid chromatographic method with amperometric
detection has been developed for the determination of the diuretic bumetanide
using a µBondapak C18 column. The mobile phase consists of a 50:50 acetonitrile–water
mixture containing 5mM KH2PO4–K2HPO4 (pH 4.0). The compound is monitored
at +1350 mV with an amperometric detector equipped with a glassy carbon working
electrode. A liquid–liquid or solid–liquid extraction is done prior
to chromatographic analysis in order to avoid the interferences found in the
urine matrix. The percentages of recovery obtained are 71% ± 1% for liquid–liquid
extraction and 84.2% ± 0.7% for solid–liquid extraction. The method
developed has a linear concentration range from 50 to 499 ng/mL with a reproducibility
in terms of relative standard deviation of 1.73% and 3.85% for a concentration
level of 70 ng/mL and 237 ng/mL, respectively, and a detection limit of 0.25
ng/mL (3:1 signal-to-noise ratio). The method is applied to the determination
of bumetanide in pharmaceutical formulations and urine obtained from hypertensive
patients and healthy volunteers after the ingestion of a therapeutic dose of
Fordiuran (1 mg bumetanide).
Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
This
article is available in its entirety by fax for $4.00 per
page.
Visa or MasterCard accepted. To
order electronically
click here
or call: 847-647-2900 ext. 1323
or fax request to: 847-647-1155.
Please
indicate JCS volume and issue along with
page numbers. |
Site Map: Home
| Current Issue | Subscribe
| Back Issues | About
Us | Meetings | Advertising
|
| Books for Sale | For
the Author | Links | Supplier
Info | Search | |
