Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
39, Number 10, October 2001, pp. 405-410
Simultaneous Determination of Gold and Copper by Reversed-Phase
High-Performance Liquid Chromatography Using Online Column Enrichment as Their
syn-Phenyl-2-Pyridylketoximate Chelates
Susumu Ichinoki*, Takehiko Okutani, Naoaki Konakawa, Takayoshi
Nakano, and Youichi Fujii
Faculty of Pharmaceutical Sciences, Hokuriku University, Ho 3, Kanagawa-machi,
Kanazawa, 920-1181, Japan
Gold and copper ions react with syn-phenyl-2-pyridylketoxime
(PPKO) at 40°C to form neutral chelates. Metal–PPKO chelates subsequently
become preconcentrated on a minicolumn packed with a divinylbenzene–methacrylate
copolymer. By switching the valve, these chelates are separated on the silica-based
phenyl column and detected with a photometric detector. These processes occur
automatically except for chelation. The results of the chelation, preconcentration,
and separation conditions studies are presented. Calibration curves for Au and
Cu ions are linear from nanograms per milliliter (parts per billion) to micrograms
per milliliter (parts per million) in the original solution. The precision for
0.5-ppm standards of Au and Cu is 2.1 and 0.9 relative standard deviation (%),
respectively. The accuracy of the present method is verified for Au and Cu based
on the analysis of a standard alloy of the National Institute of Standards and
Technology (NIST). The limits of detection for Au and Cu are 16.7 and 0.6 ppb,
respectively. The effects of foreign ions on the determination of Au and Cu
are discussed.
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