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Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
39, Number 4, January 2001, pp. 487-490
Fast Generic-Gradient Reversed-Phase High-Performance Liquid
Chromatography Using Short Narrow-Bore Columns Packed with Small Nonporous Silica
Particles for the Analysis of Combinatorial Libraries
F. Leroy, B. Presle, F. Vérillon,
and E. Vérette
Gilson S.A.S., 19, avenue des Entrepreneurs, 95400 Villiers
le Bel, France
The extremely large number of samples generated for the quality
control analysis of combinatorial libraries that are developed in the pharmaceutical
research for drug discovery requires fast generic methods such as rapid-gradient
reversed-phase high-performance liquid chromatography (HPLC). These methods
are necessary as standard procedures to produce up to several hundreds of analytical
results per day and should be optimized in order to be applied to library products
of widely differing polarities. This work presents an optimized generic method
using a narrow-bore column packed with 1.5-µm nonporous particles and
a completely automated HPLC workstation configured for the best efficiency,
throughput, and robustness with this column. A test mix of 12 compounds with
a wide polarity range is separated within 1.5 min with a cycle time of 3.5 min.
The throughput is further enhanced using a Gilson 233XL dual-injection sampler
to feed two parallel HPLC systems in order to perform 34 analyses per hour.
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