Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
39, Number 4, January 2001, pp. 468-472
An Empirical Approach for Estimating the Equivalent Chain Length
of Fatty Acid Methyl Esters in Multistep Temperature-Programmed Gas Chromatography
Supathra Lomsugarit*, Jirasak
Katsuwon*, Narumon Jeyashoke, and Kanit Krisnangkura Biochemical Technology Division, King Mongkut’s University
of Technology Thonburi, Suksawasdi 48, Toongkru, Bangkok 10140, Thailand
The equivalent chain length (ECL) has been widely accepted as
an identification tool for fatty acid methyl esters (FAMEs). In this study,
FAMEs from the seed oil of Chinese mustard (Brassica juncea) analyzed on a 0.25-mm-i.d.
capillary column coated with an SA-WAX are used as an example for the calculation
of the ECL in single-, two-, three-, and four-step temperature-programmed gas
chromatography. The ECLs of unsaturated FAMEs tend to increase with temperature,
and they agree well with those reported in the literature.
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