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Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
39, Number 4, January 2001, pp. 459-462
Determination of Astragaloside IV in Radix Astragali (Astragalus
membranaceus var. monghulicus) Using High-Performance Liquid Chromatography
with Evaporative Light-Scattering Detection
Wenkui Li and John F. Fitzloff
Program for Collaborative Research in the Pharmaceutical
Sciences, Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy,
University of Illinois at Chicago, 833 South Wood Street, Chicago, IL 60612
A reverse-phase high-performance liquid chromatographic method
is developed for the determination of astragaloside IV, a characteristic constituent
in Radix Astragali. Samples are analyzed by means of a reverse-phase column
(Zorbax Eclipse XDB C18) using acetonitrile and water under gradient conditions
as the mobile phase for 30 min. An evaporative light-scattering detector is
used and set at an evaporating temperature of 43°C with a nebulizing gas
(compressed air) pressure of 3.4 bar. The detection limit (signal-to-noise ratio
> 5) of astragaloside IV is 40 ng on-column.
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