Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
39, Number 11, November 2001, pp. 453-458
Sequential
Analysis of Malic Acid and Both Enantiomers of Lactic Acid in Wine Using a High-Performance
Liquid Chromatographic Column-Switching ProcedureAlan
J. Buglass and Suk Hean Lee School of Applied Sciences, Anglia Polytechnic
University, East Road, Cambridge CB1 1PT, U.K.
A liquid chromatographic column-switching method for the sequential
determination of malic acid and both enantiomers of lactic acid in wine is described.
The procedure involves the heart cutting of lactic acid enantiomers from a reversed-phase
high-performance liquid chromatography chromatogram, retaining them, and back-flushing
them through a chiral ligand-exchange column in which they are separated. The
method is used to determine the concentration of lactic acid enantiomers in
commercial wines. The results are in satisfactory agreement with those of other
methods. The malic acid contents of various wines are also determined. The total
analysis time for one experiment is approximately 10 min.
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