Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
39, Number 10, October 2001, pp.
Preparation and Characterization of 3-(Aza-18-Crown-6) Propylsilyl
Bonded Phase for Reversed-Phase Liquid Chromatography
Shi-Lu Da, Yu-Qi Feng, Hui-Ning Da, Yin-Han Gong, and
Yuan-Wei Zhang
39,
Number 5, May 2001, pp. 183-187
Normal-Phase High-Performance
Liquid Chromatographic Determination and Identification of Aldrin, Dieldrin,
and DDTs in Eggs Naoto Furusawa
Faculty of Human Life Science, Osaka City University, Osaka 558-8585, Japan
A method for
the routine monitoring of residual aldrin, dieldrin, pp'-DDT, op'-DDT, pp'-DDE,
and pp'-DDD in eggs by high-performance liquid chromatography (HPLC) with a
photo-diode array (PDA) detector is described. The lipids extracted from a whole
egg are cleaned by a solid-phase extraction (SPE) ISOLUTE NH2 column with a
diethyl ether–hexane (5:95, v/v) eluent. The HPLC separation is carried
out using a normal-phase (LiChrosorb NH2) column, a heptane–hexane eluent
(97:3, v/v), and a PDA detector. The average recoveries from fortified target
compounds (0.1, 0.2, 0.3, and 0.4 µg/g, respectively) are 83%, with
standard deviations (SDs) between 2 and 5%. The interassay variabilities and
their SDs are 4.8% and 0.7%, with intra-assay variabilities of 2.1–3.3%.
The limits of determination for these compounds range from 0.04 to 0.08 µg/g.
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