Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
39, Number 10, October 2001, pp.
Preparation and Characterization of 3-(Aza-18-Crown-6) Propylsilyl
Bonded Phase for Reversed-Phase Liquid Chromatography
Shi-Lu Da, Yu-Qi Feng, Hui-Ning Da, Yin-Han Gong, and
Yuan-Wei Zhang
39,
Number 5, May 2001, pp. 177-182
Analysis of Endosulfan
and Its Metabolites in Human Serum Using Gas Chromatography–Tandem Mass
Spectrometry
F.J. Arrebola[1], J.L. Martínez Vidal[1], and A. Fernández-Gutiérrez[2]
[1] Department of Analytical Chemistry, University of Almería, 04071
Almería, Spain
[2] Department of Analytical Chemistry, University of Granada, 18071 Granada,
Spain
A
new analytical method combining solid-phase extraction and gas chromatography–tandem
mass spectrometry (GC–MS–MS) is proposed to determine the insecticide
endosulfan as well as its metabolites endosulfan-ether, -lactone, and -sulfate
in human serum. Most matrix interferences are avoided using a cleanup step included
in the sample treatment and an instrumental technique such as GC–MS–MS,
which presents a high sensitivity and selectivity. Recoveries of spiked compounds
range between 94.8 and 100.4% and 93.4 and 99.7% at fortification levels of
10 and 30 ng/mL, respectively. The relative standard deviation is lower than
17.6% in all cases, and the limits of detection calculated range from 6 to 19
pg/mL. Serum samples of nine agricultural workers that spray endosulfan into
greenhouses in Almería (Spain) and two nonoccupationally exposed people
are analyzed, and endosulfan is found in all the samples studied.
Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
This
article is available in its entirety by fax for $4.00 per
page.
Visa or MasterCard accepted.
To
order electronicallyclick here or call: 847-647-2900 ext. 1323 or fax request to: 847-647-1155.
Please
indicate JCS volume and issue along with
page numbers.
