Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
39, Number 3, March 2001, pp. 87-92
High-Performance
Liquid Chromatographic Analysis for the Characterization of Triterpenoids from
Ganoderma
Ching-Hua
Su[1], Yi-Zhen Yang[1], Hsiu-O Ho[2], Chung-Hong Hu[1], and Ming-Thau Sheu[2]
[1]Center for Biotechnical Development and Research
[2]Graduate Institute of Pharmaceutical Sciences, Taipei Medical University,
250 Wu-Hsing Street, Taipei, Taiwan, R.O.C.
A high-performance
liquid chromatographic (HPLC) analysis of triterpenoids from Ganoderma is developed
and validated in an attempt to explore a way to differentiate a number of species
of the genus Ganoderma. Results show that 64 samples examined in this study
could be divided into 18 groups based on characteristics of the HPLC pattern
of triterpenoids. This result also conforms with those of the morphological
examination and the interfertility test by di-monokaryotic mating. The HPLC
analysis of triterpenoids further reveals that differentiation among samples
from three different regions each of the two species G. lucidum and G. tsugae
is workable. Even then, an incorrect designation is found for two of the groups
of samples that were originally classified as G. resinaceum but showed different
morphological characteristics and mating incompatibility. In conclusion, an
HPLC analysis of triterpenoids is a simple and easy way to differentiate among
different species of the genus Ganoderma.
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