Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
39, Number 3, March 2001, pp. 121-124
Determination
of Carvedilol in Human Cardiac Tissue by High-Performance Liquid Chromatography
Frederike
Behn, Stephanie Läer, and Hasso Scholz
Institut für Experimentelle und Klinische Pharmakologie und Toxikologie,
Abteilung für Pharmakologie, Universitätsklinikum Hamburg-Eppendorf,
Martinistrasse 52, 20246 Hamburg, Germany
A new high-performance
liquid chromatographic method has been developed for the determination of the
b-receptor blocker carvedilol in human cardiac tissue. After homogenizing tissue
samples in a microdismembrator, carvedilol and the internal standard naftopidil
are extracted with acetone. The extract is evaporated to dryness and reconstituted
in a potassium acetate buffer of pH 3.5. Samples are cleaned up with solid-phase
extraction columns. Carvedilol and the internal standard show recoveries of
69.8 ± 12.2% and 63.9 ± 9.34%, respectively. The linearity range
for carvedilol is 0.01–0.35 ng/mg (parts per billion) tissue (wet weight),
and the limit of quantitation is 0.01 ng/mg. The percentage coefficient of variation
of the intra-assay varies between 1.45 and 5.38% and the interassay between
4.25 and 6.96%. To use as an application of the assay, the cardiac carvedilol
tissue level in a patient on oral carvedilol therapy for congestive heart failure
is reported.
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