Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
39, Number 6, June 2001, pp. 251-254
Simultaneous
Determination of p-Hydroxybenzaldehyde, p-Hydroxybenzyl Alcohol, 4-(b-d-Glucopyranosyloxy)-Benzyl
Alcohol, and Sugars in Gastrodia elata Blume Measured as Their Acetylated Derivatives
by GC–MSHong-xia
Li, Ming-yu Ding*, and Jian-yuan Yu
Chemistry Department of Tsinghua University, Beijing 100084, China
A method for the simultaneous separation and determination of
the active constituents and three sugars in the roots of Gastrodia elata Blume
(GE), which is used as a famous Chinese traditional herbal medicine, by gas
chromatography–mass spectrometry is established. The samples are acetylated
with pyridine–acetic anhydride. The contents of 4-hydroxybenzaldehyde,
4-hydroxybenzyl alcohol (HA), fructose, glucose, 4-(b-d-glucopyranosyloxy)-benzyl
alcohol (GA), and sucrose in GE are 0.004%, 0.03%, 1.36%, 1.12%, 1.97%, and
4.25%, respectively, and the detection limits are 1.5, 3.0, 11.0, 5.0, 33.0,
and 35.0 pg, respectively. The contents of HA and GA in the urine and brain
of a mouse are also determined. This method is simple, reliable, and quick for
the simultaneous determination of the active constituents and sugars in GE.
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