Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
39, Number 6, June 2001, pp. 217-221
High-Performance
Liquid Chromatography Coupled with Chemiluminescence Nitrogen Detection for
the Study of Ethoxyquin Antioxidant and Related Organic BasesD.
Brannegan, M. Ashraf-Khorassani, and L.T. Taylor*
Department of Chemistry, Virginia Tech, Blacksburg, VA 24061-0212
The Chemiluminescent Nitrogen Detector (CLND) for use with high-performance
liquid chromatography (HPLC) allows for the low-level detection of nitrogen-containing
compounds with simple quantitation. The nitrogen selective detector’s equimolar
response (i.e., equal response for nitrogen independent of its chemical environment)
allows for any nitrogen-containing compound to be quantitated as long as the
number of nitrogens are known. The HPLC–CLND provides a new detection method
for analytes that are not available in large quantities or have unknown chemical
or physical characteristics such as oxidation products, metabolites, or impurities.
Ethoxyquin is a primary antioxidant that is used to preserve many food products
and animal feeds. HPLC–CLND is used in the study of the oxidation products
of ethoxyquin because limited quantities of these compounds are available and
subsequent calibration curves are difficult to maintain. HPLC–CLND as a
new method of detection has been evaluated for its equimolarity of response,
linear range, limit of detection, and limit of quantitation.
Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
This
article is available in its entirety by fax for $4.00 per
page.
Visa or MasterCard accepted.
To
order electronicallyclick here or call: 847-647-2900 ext. 1323 or fax request to: 847-647-1155.
Please
indicate JCS volume and issue along with
page numbers.
