Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
39, Number 7, July 2001, pp. 280-286
Preparation
of a Ribonucleic Acid–(Polyamidoamine)– (Zirconia–Urea-Formaldehyde
Resin) High-Performance Liquid Affinity Chromatographic Stationary PhaseShulei
Lei, Shilin Yu*, and Chunfeng Zhao
College of Science, Beijing University of Chemical Technology, 15 Beisanhuan
East Road, Chaoyang District, Beijing 100029, China
A preparative
method for a high-performance liquid affinity chromatographic (HPLAC) stationary
phase is described. The 3- to 5-µm nonporous composite spherical microparticles
of zirconia and urea-formaldehyde (UF) resin are synthesized through the reaction
of zirconyl chloride with hexamethylene tetra-amine and urea, and then it is
used as the matrix of the HPLAC stationary phase of which the diameter and structure
are determined by scanning electron microscopy. In a methanol medium, the polyamidoamine
(PAMAM) starburst dentritic spacer arms are linked with the imido-groups on
the surface of the matrix by the Michael addition reaction with methyl acrylate
and the amination reaction with ethylene diamine. After repeating these steps
in triplets, amine-terminated dentritic spacer arms with a generation of 3 are
obtained. The topological structure of the spacer arms is examined by solid-state
13C NMR. The Br-substituted ribonucleic acid (RNA) ligand is obtained by the
reaction of liquid bromine with RNA and bonded to the dendritic spacer arms
of the matrix in a solution of NaOH (pH 9–11). The binding capacity of
RNA is measured by UV spectrophotometry. A new type of stationary phase—RNA–(PAMAM)–(zirconia–UF
resin—for HPLAC, which possesses starburst dendritic spacer arms, is synthesized
and used for the separation of biological macromolecules.
Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
This
article is available in its entirety by fax for $4.00 per
page.
Visa or MasterCard accepted.
To
order electronicallyclick here or call: 847-647-2900 ext. 1323 or fax request to: 847-647-1155.
Please
indicate JCS volume and issue along with
page numbers.
