Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
39, Number 7, July 2001, pp. 269-272
Fatty
and Resin Acid Analysis in Tall Oil Products via Supercritical Fluid Extraction–Supercritical
Fluid Reaction Using Enzymatic CatalysisScott
L. Taylor* and Jerry W. King
Food Quality and Safety Research Unit, National Center for Agricultural Utilization
Research, Agricultural Research Service, U.S. Department of Agriculture, 1815
N. University Street, Peoria, IL 61604
Supercritical
fluid extraction (SFE) is combined with supercritical fluid reaction (SFR) in
an analytical mode to assess tall oil products for their fatty or resin acid
content or both. The SFR consists of an inline enzymatically catalyzed reaction
in which a lipase transesterifies specific lipids with methanol. The SFE–SFR
sequence is conducted employing commercially available extractors using supported
lipases in the extraction cell to form methyl esters. In this study, six different
commercially available lipases are screened for activity. The SFE–SFR extracts
are analyzed by capillary gas chromatography and supercritical fluid chromatography
and then compared with tall oil products derivatized by conventional chemical
derivatization techniques.
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