Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
39, Number 7, July 2001, pp. 265-268
Separation
of Inosine, Hypoxanthine, and Guanosine by High-Performance Liquid Chromatography
on SilicaH.
Kazoka
Latvian Institute of Organic Synthesis, 21 Aizkrakles str., LV-1006 Riga, Latvia
The separation
of inosine (Ino), hypoxanthine (Hyp), and guanosine (Guo) on silica has been
studied. In adsorption normal-phase systems the peak shapes are unsatisfactory;
a low selectivity has been observed for the Ino–Hyp and Guo–Hyp pairs.
Chromatograms can be significantly improved if systems with a mixed partition–adsorption
retention mechanism are applied.
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