Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
39, Number 1, January 2001, pp. 1-6
Comparison
of the Column Performance of Narrow-Bore and Standard-Bore Columns for the Chromatographic
Determination of a- b-, g-, and d-TocopherolL.
Ye, W.O. Landen, and R.R. Eitenmiller Department of Food Science and Technology, University of Georgia, Athens,
GA 30602
A comparison
of the performance of narrow-bore (2.1-mm i.d.) and standard-bore (4.6-mm i.d.)
analytical silica columns having the same length is completed for the resolution
of a-, b-, g-, and d-tocopherol.
The studies are performed on high-performance liquid chromatographic equipment
with minimum extracolumn contribution. Column permeabilities are 1.16 x 10–9
and 2.48 x 10–9 cm2 for narrow and standard bore, respectively. The narrow-bore
column gives up to a 7 times increase in sensitivity compared with a standard-bore
column at equivalent running times for the analytes. Approximately one-third
solvent savings can be achieved with the narrow-bore column. Theoretical plates
of the standard-bore column are higher than that of the narrow-bore column.
Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
This
article is available in its entirety by fax for $4.00 per
page.
Visa or MasterCard accepted.
To
order electronicallyclick here or call: 847-647-2900 ext. 1323 or fax request to: 847-647-1155.
Please
indicate JCS volume and issue along with
page numbers.
