Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
39, Number 2, February 2001, pp. 70-72
A
Liquid Chromatographic Method for the Simultaneous Determination of Promethazine
and Three of Its Metabolites in Plasma Using Electrochemical and UV Detectors
Sreenivasa
R. Vanapalli[1], Sivarama P. Kambhampati[1], Lakshmi Putcha[2], and David W.A.
Bourne[1]
[1]College of Pharmacy, University of Oklahoma Health Sciences Center, Oklahoma
City, OK 73117
[2]Life Sciences Research Laboratories, Mail Code SD3, NASA Johnson Space Center,
Houston, TX 77058
A new assay
method has been developed for the quantitation of promethazine (PMZ) with a
sensitivity and reproducibility as good as any previously reported method. This
method is also capable of quantitatively determining three metabolites of PMZ
(monodemethylated, sulphoxidated, and monodemethylated sulphoxidated PMZ), which
has not been previously described. The method uses high-performance liquid chromatography
with amperometric and UV detection simultaneously and requires only one extraction
step from serum with chloroform. The method uses trifluoperazine as the internal
standard. The limit of detection level for PMZ is 1.0 ng/mL when a 0.2-mL specimen
of plasma is assayed. A validation study is also conducted for evaluating the
recovery, precision, linearity of response, sensitivity, and selectivity of
the method.
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