Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
39, Number 2, February 2001, pp. 49-53
Determination
of Bromethalin in Commercial Rodenticides Found in Consumer Product Samples
by HPLC–UV–vis Spectrophotometry and HPLC–Negative-Ion APCI–MS
Mantai
Z. Mesmer and Rick A. Flurer
United States Food and Drug Administration, Forensic Chemistry Center, 6751
Steger Drive, Cincinnati, OH 45237-3097
A small amount
of green particulate material is encountered in a consumer complaint sample.
The green particulates in the sample are identified as a bromethalin-containing
rodenticide using high-performance liquid chromatographic (HPLC)–UV–vis
spectrophotometric and HPLC–negative-ion atmospheric pressure chemical
ionization (APCI)–mass spectrometric (MS) approaches, which are commonly
used for the detection and confirmation of bromethalin in grain-based rodenticides.
The selective and sensitive nature of the MS detector makes it possible to determine
bromethalin without extensive sample cleanup and preconcentration. The estimated
detection limit with the UV–vis detector is 500 pg of bromethalin injected
into the column. The extensive fragmentation of the bromethalin molecule under
APCI conditions provides sufficient structural information for positive identification.
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