Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
39, Number 12, December 2001, pp. 508-512
A Direct High-Performance Liquid Chromatographic Method for the
Determination of Therminol 66 Contamination in Commercial Glycerin and Fatty
Acids
M.H. Moh[1], T.S. Tang[1],
and G.H. Tan[2] [1]Advanced Oleochemical Technology Center, Malaysian Palm
Oil Board, No. 6 Persiaran Institusi, Bandar Baru Bangi, 43650 Kajang, Selangor,
Malaysia
[2]Department of Chemistry, University of Malaya, Lembah Pantai, 50603 Kuala Lumpur,
Malaysia
A simple and sensitive high-performance liquid chromatographic
method for the determination of Therminol 66 thermal heating fluid in glycerin
and fatty acids is developed. Sample solutions dissolved in methanol–tetrahydrofuran
(50:50, v/v) are injected directly into a reversed-phase C18 column and eluted
with a methanol and water mixture (88:12, v/v). The concentration of the thermal
heating fluid is monitored by fluorescence detection at 257 nm (excitation)
and 320 nm (emission). The calibration graph obtained from various concentrations
of the thermal heating fluid in the methanol and tetrahydrofuran mixture is
linear (correlation coefficient = 0.999), and the limit of detection is 0.01
µg/mL. Spiked glycerin containing 0.1 to 1.0 µg/g of the thermal
heating fluid also gives good linearity with a mean recovery of 95.3%. The mean
intra- and interassay precision are 1.80–6.51% and 5.71–9.03%, respectively,
at the 0.1-µg/g level. The method is simple and does not require any pretreatment
step, thus it is ideal for quality assurance purposes.
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