Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
39, Number 8, August 2001, pp. 339-344
A
Simplex-Optimized Chromatographic Separation of Fourteen Cosmetic Preservatives:
Analysis of Commercial ProductsE.
Marengo*, M.C. Gennaro, and V. Gianotti Dipartimento di Scienze e Tecnologie Avanzate, Università del Piemonte
Orientale Amedeo Avogadro, Corso Borsalino, 54, 15100 Alessandria, Italy
An
ion-interaction high-performance liquid chromatography (HPLC)–diode-array
detection method is developed and optimized for the separation of typical antimicrobial
agents used in cosmetics and hygiene products. The most used preservatives contain
different molecular structures, different functionalities, and are characterized
by different chemical properties. Organic acids, alkyl esters of benzoic acids,
alkyl p-hydroxy benzoic acids (parabens), phenol derivatives, and carbanilides
represent the most used preservatives, and are often present in multicomponent
mixtures. In order to develop a multicomponent method to be used in quality
control analysis, the ion-interaction reagent reversed-phase HPLC technique
seems to be particularly suitable, because it allows for the simultaneous separation
of acidic, basic, and neutral species. The experimental conditions of the method
are developed by OVAT (one variable at a time) treatment and further optimized
by a multivariate approach based on a Simplex algorithm that works on a desirability
function targeted to maximize the resolution in a multicomponent mixture. The
new method proposed that is able to simultaneously separate fourteen preservatives
is applied in the analysis of commercial products.
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