Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
38, Number 9, September 000, pp. 383-385
High-Performance
Liquid Chromatographic Determination of Glycogen in Sea Urchin Gonads with Refractive
Index DetectionJ.
López-Hernández*, M.J. González-Castro, and M. Pereda-González Nutrition and Bromatology Group, Analytical Chemistry, Nutrition and
Bromatology Department, Pharmacy Faculty, University of Santiago de Compostela,
E-15706 Santiago de Compostela, La Coruña, Spain.
A high-performance
liquid chromatographic method using refractive index detection for the determination
of glycogen in sea urchin (Paracentrotus lividus) gonads is developed. After
alkaline digestion with sodium carbonate, samples are adjusted to pH 4.6 with
citric acid and incubated with amyloglucosidase to hydrolyze the glycogen. The
resulting glucose is determined using a Spherisorb NH2 column as the stationary
phase and an acetonitrile–water mixture (80:20, v/v) as the mobile phase.
The relative standard deviation (%) was 3.57, the limit of detection was 40.1
µg/mL, and the recovery percentage was 97.2%.
Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
This
article is available in its entirety by fax for $40.00.
Visa, MasterCard & Amex accepted.
To
order electronicallyclick here or call: 847-647-2900 ext. 1323 or fax request to: 847-647-1155.
