Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
38, Number 10, October 2000, pp. 450-457
Comparative
Evaluation of Four Detectors in the High-Performance Liquid Chromatographic
Analysis of Chiral Nonaromatic Alcohols
B.
Toussaint1, A.L.L. Duchateau2, S. van der Wal2, A. Albert3, P. Hubert1, and
J. Crommen1 1Department of Analytical Pharmaceutical Chemistry, Institute
of Pharmacy, University of Liège, CHU, B 36, B-4000 Liège, Belgium;
2DSM Research, P.O. Box 18, 6160 MD Geleen, The Netherlands; and 3Department
of Biostatistics, University of Liège, CHU, B 23, B-4000 Liège,
Belgium
A comparative
evaluation of ultraviolet, polarimetric, refractive index, and evaporative light-scattering
detection coupled with high-performance liquid chromatography has been developed
for the separation and quantitation of the enantiomers of chiral nonaromatic
alcohols, some of which are intermediates in the synthesis of chiral drugs.
(R,S)-3-tert-butylamino-1,2-propanediol; (R,S)-glycidol; and (R,S)-1-(4-morpholino)-2-octanol
are selected as model compounds in order to compare the detection sensitivity
and the linearity of the response with the four detectors. Separation of the
enantiomers is performed using chiral stationary phases in normal-phase liquid
chromatography. A one-day validation is achieved for (S)-3-tert-butylamino-1,2-propanediol
with each detector, and limits of quantitation are determined for the three
compounds. Advantages and limitations of the four detectors are discussed.
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