Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
38, Number 10, October 2000, pp. 425-429
Deconvolution
of Composite Chromatographic Peaks by Simultaneous
Dual Detections András
Gergely, Péter Horváth, and Béla Noszál* Institute of Pharmaceutical Chemistry, Semmelweis University, Högyes
E. u. 9., H-1092 Budapest, Hungary
Composite
chromatographic peaks are deconvoluted by a method that uses ratio formation
from signals of simultaneous double detection. The method is generally suitable
if two detector signals can simultaneously be acquired and their uses do not
need any a priori assumption or mathematical shape analysis. A simple deduction
makes the compound- and detector-specific intensive parameters explicit, which
allows for the digital construction of directly invisible component peaks. The
simultaneous double detection is shown to be superior to coupled detectors,
sequentially fixed chromatograms, and subsequently synchronized peaks. The combination
of circular dichroism and ultraviolet (UV) detection is shown to be especially
advantageous in the analysis of enantiomers for which the other ratio-forming
peak-deconvolution techniques have inherently been insensitive. The double chiroptical
UV detection can be of further use to decompose overlapping peaks of nonenantiomeric
compounds that are highly similar. The capacity of the method is exemplified
by the analysis of morphine alkaloids, steroid oximes, and synthetic heterocycles.
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