Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
38, Number 10, October 2000, pp. 421-424
Separation
and Identification of Volatile Components in the Fermentation Broth of Trichoderma
atroviride by Solid-Phase Extraction and Gas Chromatography–Mass Spectrometry
Ágnes
Keszler*, Esther Forgács, and László Kótai Institute of Chemistry, Chemical Research Center, Hungarian Academy
of Sciences, H-1525 Budapest, P.O. Box 17, Hungary Juan Antonio Vizcaíno,
Enrique Monte, and Isabel García-Acha Department of Microbiology and
Genetics, CSIC/University of Salamanca, Salamanca, Spain
A preseparated
fermentation broth of Trichoderma atroviride strain 11 is analyzed by gas chromatography
followed by mass-spectral detection using a Finnigan MAT GCQ apparatus. After
preseparation in a C18 and a silica gel column, nineteen pyrone and dioxolane
derivatives and two aliphatic esters are obtained, respectively. Among these,
the four dioxolane derivatives have not been identified previously. The main
component is found to be 5,5'-dimethyl-2H-pyran-2-on. The relative standard
deviation for the determination of the retention time and the peak area (measured
in ion counts) is 0.1% and 4.5%, respectively.
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