Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
38, Number 11, November 2000, pp. 489-495
Application
of Convective Interaction Media (CIM®) Disk Monolithic Columns for Fast
Separation and Monitoring of Organic Acids Martina
Vodopivec1, Ales Podgornik2,*, Marin Berovic3, and Ales Strancar2
1National Institute of Chemistry, Hajdrihova 19, c; 2BIA Separations d.o.o.,
Teslova 30; and 3Department of Chemical,
Biochemical and Ecological Engineering, University of Ljubljana, Hajdrihova
19, SI-1000 Ljubljana, Slovenia
The separation
of organic acids on the anion-exchange monolithic support, commercially available
as Convective Interaction Media (CIM), is presented in this study. It is demonstrated
that citric, isocitric, pyruvic, fumaric, malic, and a-ketoglutaric acid can
be successfully separated using a CIM monolithic column of suitable user-adjustable
length. The effect of the mobile phase composition on the separation is investigated.
CIM monolithic columns of adjustable length from 3 to 18 mm are compared regarding
the resolution and the back pressure. It is shown that the CIM monolithic column
of 12 mm in length enables a good separation of all six organic acids within
3 min and exhibits a linear dependence of back pressure versus flow rate. The
resolution and the dynamic binding capacity are found to be flow-unaffected.
A filtrated sample of bioprocess supernatant is analyzed without previous pretreatment,
which indicates the possibility of online monitoring of small molecules during
the bioprocess using CIM monolithic columns.
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