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Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
38, Number 5, May 2000, pp. 207-210
Elimination
of Amino Acid Interferences in the Chiral Ligand-Exchange Chromatographic Analysis
of Lactic Acid Enantiomers in Wine
Alan
J. Buglass* and Suk Hean Lee
Chiral ligand-exchange
liquid chromatography is used to identify and quantitate lactic acid enantiomers
in wines that have or have not undergone malolactic fermentation. The stationary
phase is (R)-penicillamine, which is bound lipophilically to a C18 bonded silica
matrix. The mobile phase is 1mM copper sulfate, and the detection mode is ultraviolet.
Serious interference from (S)-aspartic acid and other amino acids is eliminated
by the use of propanesulfonic acid-type cation exchange solid-phase extraction
cartridges prior to chromatographic analysis. Lactic acid enantiomers in wine
are quantitated in the range of 10 to 500 mg/L. The detection limit is 3 mg/L.
The method is also successful in the determination of lactic acid enantiomers
in certain beers (e.g., lambic beers), kim-chi, sauerkraut, and various yogurts.
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