Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
38, Number 3, March 2000, pp. 122–128
Quantitative
Analysis in Field-Flow Fractionation Using Ultraviolet–Visible Detectors:
an Experimental Design for Absolute MeasurementsAndrea
Zattoni, Dora Melucci, Giancarlo Torsi, and Pierluigi Reschiglian Department of Chemistry “G. Ciamician”,
University of Bologna, V. Selmi, 2 I-40126 Bologna, Italy
In previous
works, it has been shown that a standard ultraviolet–visible detection
system can be used for quantitative analysis of heterogeneous systems (dispersed
supermicron particles) in field-flow fractionation (FFF) by single peak area
measurements. Such an analysis method was shown to require either experimental
measurements (standardless analysis) or an accurate model (absolute analysis)
to determine the extinction efficiency of the particulate samples. In this work,
an experimental design to assess absolute analysis in FFF through prediction
of particles’ optical extinction is presented. Prediction derives from
the semiempirical approach by van de Hulst and Walstra. Special emphasis is
given to the restriction of the experimental domain of instrumental conditions
within which absolute analysis is allowed. Validation by statistical analysis
and a practical application to real sample recovery studies are also given.
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