Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
38, Number 6, June 2000, pp. 259-261
A
Simple High-Performance Liquid Chromatography Method for the Analysis of Glucose,
Glycerol, and Methanol in a BioprocessGiuseppina
Paola Parpinello and Andrea Versari
Dipartimento di Biotecnologie Agrarie ed Ambientali, Università di Ancona,
Via Brecce Bianche, 60130 - Ancona, Italy
A
reliable high-performance liquid chromatography–refractive index detection
method for the simultaneous analysis of glucose, methanol, and glycerol in a
bioprocess fermentation media using direct injection is presented. The validation
studies show a satisfactory selectivity, linearity, accuracy, and recovery of
the method. The lowest concentration detectable for glucose and methanol is
3.5 and 6.7 mg/100 mL, respectively. This method could be an attractive choice
for the analysis of these compounds not only in fermentation media but also
in biomedical and environmental samples.
Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
This
article is available in its entirety by fax for $4.00 per
page.
Visa or MasterCard accepted.
To
order electronicallyclick here or call: 847-647-2900 ext. 1323 or fax request to: 847-647-1155.
Please
indicate JCS volume and issue along with
page numbers.
