Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
38, Number 6, June 2000, pp. 234-240
Rapid
Screening of Natural Products by High-Resolution High-Temperature Gas ChromatographySilvia
Albero Carbonell, Francisco Radler de Aquino Neto, Jari Nobrega Cardoso, and
Alberto dos Santos Pereira*
LADETEC, Instituto de Química, Universidade Federal do Rio de Janeiro,
Ilha do Fundão, Cidade Universitaria, CT, Bloco A, Sala 607, Rio de Janeiro,
RJ - Brazil 21949-900 Ana Claudia F. Amaral
Lab. de Química de Produtos Naturais (LQPN), Far-Manguinhos, RJ - Brazil
21041-250 Roderick A. Barnes
Núcleo de Pesquisa de Produtos Naturais, Centro de Ciências da
Saúde, Bloco H, Universidade Federal do Rio de Janeiro, RJ - Brazil 21941-590
The
crude ethanol extracts from the leaves of three Croton hemiargyrus hemiargyreus
plants are fractionated by thin-layer chromatography, yielding five fractions.
The fractions and the crude extract are analyzed by high-temperature high-resolution
gas chromatography coupled with mass spectrometry (HT-HRGC–MS). Several
natural products, including thermolabile components, can be characterized directly
in the samples, such as alkaloids, terpenes, flavonoids, acids, alcohols, etc.
The cold on-column technique proves to be appropriate for the injection of these
thermolabile compounds. HT-HRGC–MS is shown to be a valuable tool and an
alternative technique to classical phytochemical procedures for the simple and
fast routine analysis of natural products in crude extracts.
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