Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
38, Number 6, June 2000, pp. 229-233
High-Performance
Liquid Chromatographic Analysis of Saponin Compounds in Bupleurum falcatumIn-Seon
Park, Eun Mi Kang, and Namsoo Kim*
Korea Food Research Institute, Backhyun-dong, Bundang-ku, Songnam-si, Kyungki-do
463-420, Republic of Korea
A
mixture of saponin compounds (saikosaponin c, a, and d) in the 70% ethanol extract
of a powdered sample of Bupleuri radix are analyzed by an Inertsil ODS-3 C18
column at a flow rate of 1.0 mL/min and detection wavelength of 203 nm. Well
resolved chromatograms of saikosaponin c, a, and d are obtained with a gradient
elution of acetonitrile–water from 40:60 (v/v) to 50:50 (v/v). The total
time required for a single analysis is approximately 20 min. Calibration curves
for saikosaponin c, a, and d are linear up to 2.5 mg/mL. The coefficient of
variability values for saikosaponins in the extract are below 4%, and the recoveries
for saikosaponin c, a, and d are 95.2 ± 1.1, 96.5 ± 0.9, and 96.2
± 1.0%, respectively. The changes in saikosaponin contents for a two-year
growth of Bupleurum falcatum are measured by the established high-performance
liquid chromatography method.
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