Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
38, Number 7, July 2000, pp. 289-296
Using
Microwave Distillation-Solid-Phase Microextraction–Gas Chromatography–Mass
Spectrometry for Analyzing Fish TissueCasey
C. Grimm, Steven W. Lloyd, Rebecca Batista, and Paul V. Zimba 1USDA-ARS-SRRC, 1100 Robert E. Lee Blvd., New Orleans,
LA 70124 and 2USDA-ARS-MSA, Catfish Genetics Research Unit, Thad Cochran National
Warmwater Aquaculture Center, Stoneville, MS 38776
A technique
for the analysis of the volatile compounds from fish tissue employing microwave
distillation-solid-phase microextraction–gas chromatography–mass spectrometry
is described. A qualitative listing of 174 compounds observed in the headspace
is given, and a quantitative method for the determination of the off-flavor
contaminants (2-methylisoborneol and geosmin) is presented. Borneol and decahydro-1-naphthol
are used as the surrogate and internal standards, respectively. A linear calibration
curve is obtained for 0.1 to 5 ppb with a recovery level of 60% at 2.5 ppb.
Comparison of the instrumental method with a human flavor checker showed good
agreement.
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