A High-Performance Liquid Chromatographic Method for the Determination of Monofluoroacetate

Published: Journal of Chromatographic Science, ISSN 0021-9665 Volume 38, Number 1, January 2000, pp. 16-20

A High-Performance Liquid Chromatographic Method for the Determination of Monofluoroacetate
P.P. Minnaar and G.E. Swan
Department of Pharmacology and Toxicology, University of Pretoria, P/Bag X04, Onderstepoort, 0110, South Africa
R.I. McCrindle and W.H.J. de Beer
Department of Chemistry and Physics, Technikon Pretoria, P/Bag X680, Pretoria, 0001, South Africa
T.W. Naudé
Division of Toxicology, Onderstepoort Veterinary Institute, P/Bag X05, Onderstepoort, 0110, South Africa

A simple isocratic high-performance liquid chromatographic (HPLC) method for the quantitative analysis of monofluoroacetic acid (MFA), the toxic substance of Dichapetalum cymosum, in plant material, rumen contents (gastric contents), and liver samples is described. A suitable HPLC column that gives optimum sensitivity, accuracy, precision, and separation of MFA is identified. A C-610 organic acid analysis column at ambient temperature with 0.02M H₃PO₄ as an eluent and ultraviolet detection at 210 nm is utilized to quantitate MFA. Using this method, the average percentage recovery in plant material, bovine liver, and rumen samples is 94.8%, and a detection limit of 12 µg/L is achievable.

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