Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
38, Number 2, February 2000, pp. 77–83
Analyte
Loss Due to Membrane Filter Adsorption as Determined by High-Performance Liquid
ChromatographyM.
Carlson and R.D. Thompson Department of Health and Human Services, U.S. Food and Drug
Administration, 240 Hennepin Avenue, Minneapolis, MN 55401
The
phenomenon of membrane filter adsorption in high-performance liquid chromatography
(HPLC) is investigated utilizing 16 brands of filters representing 3 polymeric
materials: cellulose acetate (CA), nylon, and polyvinylidene difluoride in a
variety of diameters (3, 4, 7, 13, and 25 mm). Sixteen compounds commonly encountered
in drug preparations are selected as sample analytes and classified as acidic,
basic, and neutral in chemical behavior. Six mobile phase/sample solvent mixtures
are included: 3 with methanolÐwater and 3 with acetonitrileÐwater as major constituents.
When using methanol as the mobile phase organic component, CA, nylon, and polyvinylidene
difluoride (PVDF) filters exhibit negligible to moderate adsorption levels with
regard to the neutral and basic drug compounds. The acidic drug test compounds
are adsorbed by 50% of all 3 filter materials tested in methanolÐwater. In acetonitrile,
neutral compounds are affected by 31.4%, basic compounds are affected by 47.0%,
and acidic compounds are affected by 53.6% of the nylon and PVDF filters. CA
is incompatible with acetonitrile and is excluded from the study with this solvent.
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