Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
38, Number 2, February 2000, pp. 72–76
Liquid
Chromatographic Determination of Rifampin In Human Plasma, Bronchoalveolar Lavage
Fluid, and Alveolar CellsJohn
E. Conte, Jr., Emil Lin, and Elisabeth Zurlinden Department of Epidemiology and Biostatistics, Infectious
Diseases Research Laboratory, Department of Medicine, Department of Microbiology
and Immunology, and Department of Biopharmaceutical Sciences, University of
California, San Francisco, San Francisco, CA
A
technique is presented for the measurement of rifampin in humans by reversed-phase
column chromatography and postcolumn photo irradiation. The assay employs diazepam
as an internal standard and provides specific, rapid, and reliable determinations
for drug concentration in plasma, bronchoalveolar lavage (BAL), and alveolar
cells (AC). The preparation of plasma and AC samples utilizes a simple deproteinization
step, whereas BAL supernatants require a solid-phase extraction. The assay produces
sharp peaks with retention times of 6.2 and 15 min for rifampin and diazepam,
respectively. The detection limits for rifampin were 0.5 µg/mL for plasma, 0.015
µg/mL for BAL supernatants, and 0.03 µg/mL for AC suspensions. The assay has
excellent performance characteristics, making it suitable for pharmacological
studies in humans, and it is being used to support a study of the intrapulmonary
pharmacokinetics of rifampin.
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