Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
38, Number 2, February 2000, pp. 67–71
A
Convenient Method for Routine Estimation of Dead Time in Gas ChromatographyTasanee
Watanachaiyong, Narumon Jeyashoke, and Kanit Krisnangkura School of Bioresources and Technology, King Mongkut's University
of Technology, Thonburi, Bangkok 10140, Thailand
A
set of retention times (tR) of n-alkanes at different
temperatures and the primary dead times (tM) are used to determine
the 4 numerical constants (a, b, c, and d) of an equation. This
equation is rearranged into a second equation, used in turn for routine calculation
of the secondary dead time (tMS) of each chromatogram from
any member of the n-alkane series. Equation 2 can be used to calculate
the tMS of both packed and capillary columns. The calculated tMS values are
in good agreement with those of the tM values. The greatest
difference is 3.38%, but it is speculated that the percent difference would
be lower when more tR data are collected for the determination
of the 4 numerical constants of Equation 1. Error in measurement of retention
time undoubtedly would affect the accuracy of the estimated dead time, but it
is attenuated by a factor of 1 + e(a + bN + c/T
+ dN/T).
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