Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
38, Number 12, December 2000, pp. 545-550
Capillary
Zone Electrophoresis with Electrochemical Detection for the Determination of
Glutathione in Human Red Blood Cells without Preseparation of Hemoglobin
Wenrui
Jin[1], Wei Li[2], and Qiang Xu[3]
[1]Laboratory of Analytical Science, School of Chemistry, Shandong University,
Jinan 250100, China
[2]Health and Diseases Preventive Centre of Shandong Province, Jinan 250014,
China; and
[3]Animal Husbandry Bureau of Shandong Province, Jinan 250012, China
Interference
is studied for the determination of glutathione (GSH) in human red blood cells
by using capillary zone electrophoresis with end-column amperometric detection
at a gold–mercury amalgam microelectrode. It is found that when interference
substances such as hemoglobin (Hb) in the hemolysate flow off from the end of
the separation capillary, they can be adsorbed on the surface of the electrode
and interfere with the signal of GSH. If the concentration of hemolysate is
lower than 0.5% (v/v), this phenomenon can be overcome because they are adsorbed
on the surface of the capillary wall and do not flow off from the capillary.
A method is developed for the determination of GSH in human erythrocytes without
the preseparation of Hb.
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