Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
38, Number 12, December 2000, pp. 535-544
Ultrafast
Reversed-Phase High-Performance Liquid Chromatographic Separations: An Overview
J.J.
Kirkland
Agilent Technologies, Chemical Analysis Group, Newport Site, 538 First State
Boulevard, Newport, DE 19804
Ultrafast
reversed-phase high-performance liquid chromatographic (HPLC) separations are
often needed for analyses related to combinatorial chemistry, studies in liquid
chromatography–mass spectrometry, and other applications in which very
rapid sample turnaround is paramount. Unfortunately, no consensus exists regarding
the best column technology for optimally performing the desired rapid separations.
This overview compares the advantages and limitations for columns of ultramicroporous,
ultramicrononporous, and superficially porous particles and monolith structures
for the very fast separation of solutes by reversed-phase HPLC. Data from literature
and the author’s laboratory are used to illustrate the strengths and limitations
of the various approaches that can be used for ultrafast separations.
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