Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
38, Number 8, August 2000, pp. 325-328
A
Precolumn Derivatization High-Performance Liquid Chromatographic Method with
Improved Sensitivity and Specificity for the Determination of Astragaloside
IV in Radix AstragaliMeicun
Yao, Ying Qi, Kaishun Bi, Xi Wang, and Xu Luo
Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110015, P.R. China
Chuntao Che
Department of Chemistry, Hong Kong University of Science & Technology, School
of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories,
Hong Kong
A
reversed-phase high-performance liquid chromatographic method is developed for
the determination of astragaloside (AGS) IV, which is known as the active constituent
of Radix Astragali. The method uses precolumn derivatization with benzoyl chloride
to form the benzoyl ester of AGS IV quantitatively and is carried out with a
wide-ranging concentration (0.004–0.080 mg/mL) of the derivatized AGS IV.
The eluent consists of 90% methanol, 4% tetrahydrofuran, 6% water, and 0.2%
triethylamine, with vitamin D3 added as the internal standard.
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