Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
38, Number 4, April 2000, pp. 169–173
High-Performance
Liquid Chromatography–Electrospray Ionization-Mass Spectrometry Study of
Ginkgolic Acid in the leaves and Fruits of the Ginkgo Tree (Ginkgo biloba)Xian-guo
He, Matthew W. Bernart, Gary S. Nolan, Long-ze Lin, and Michael P. Lindenmaier Research Laboratory of Natural Products Chemistry,
A.M. Todd Botanicals, 4091 W. 11th Avenue, Eugene, OR 97402
A method is
developed for qualitative analysis of ginkgolic acids in the leaves and fruits
of Ginkgo biloba by high-performance liquid chromatography (HPLC)–electrospray
ionization-mass spectrometry technique. Negative ionization mode is successful
in obtaining a very abundant deprotonated molecule [M – H]–.
The mass detection sensitivity is higher than ultraviolet detection but relies
heavily on the concentration of acetic acid in the HPLC eluent, which consists
of acetonitrile–water–acetic acid. The method is also very specific
for the analysis of ginkgolic acid with no interferences from the sample matrix.
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