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Article Abstracts

Published:Journal of Chromatographic Science, ISSN 0021-9665Volume 38, Number 4, April 2000, pp. 169–173

High-Performance Liquid Chromatography–Electrospray Ionization-Mass Spectrometry Study of Ginkgolic Acid in the leaves and Fruits of the Ginkgo Tree (Ginkgo biloba) Xian-guo He, Matthew W. Bernart, Gary S. Nolan, Long-ze Lin, and Michael P. Lindenmaier
Research Laboratory of Natural Products Chemistry, A.M. Todd Botanicals, 4091 W. 11th Avenue, Eugene, OR 97402

A method is developed for qualitative analysis of ginkgolic acids in the leaves and fruits of Ginkgo biloba by high-performance liquid chromatography (HPLC)–electrospray ionization-mass spectrometry technique. Negative ionization mode is successful in obtaining a very abundant deprotonated molecule [M – H]. The mass detection sensitivity is higher than ultraviolet detection but relies heavily on the concentration of acetic acid in the HPLC eluent, which consists of acetonitrile–water–acetic acid. The method is also very specific for the analysis of ginkgolic acid with no interferences from the sample matrix.

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