Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
38, Number 4, April 2000, pp. 157–161
Temperature
Effects on Packed-Capillary Liquid Chromatography of the X-ray Contrast Agent
IodixanolP.
Molander1, M. Theodorsen1, E. Lundanes1, D.
Malthe Soerenssen2, and T. Greibrokk1 1Department of Chemistry, University of Oslo, P.O. Box
1033 Blindern, 0315 Oslo, Norway and 2Nycomed Imaging ASA, 4510 Spangereid,
Norway
The effect
of varying the operating temperature from 6 to 90°C on the chromatographic
performance of the exo–exo and exo–endo isomers of the
X-ray contrast agent Iodixanol in packed-capillary reversed-phase liquid chromatography
shows increasing interconversion rates between the two isomeric conformers with
increasing temperature. At 90°C, Iodixanol elutes as one sharp peak due
to an increased interconversion rate between the two isomeric conformers. Consequently,
increased sensitivity is achieved. Temperature programming from 6 to 40°C
is utilized to optimize the resolution and determination of the exo–exo
and exo–endo isomers. Temperature programming provides a significant
decrease in the retention times in comparison with the isothermal separations
while still preserving baseline separation of the isomers.
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